Nex-Gen Core: Next Generation Sequencing Core Facility

UTMB’s Next Generation Sequencing (NGS) Core utilizes instrumentan Illumina HiSeq 1000 sequencing system to perform massively parallel sequencing for genetic analysis. The NGS Core offers support in library construction from various template sources; RNA (total, poly A+ and miRNA), chromatin immunoprecipitated DNA (ChIP-Seq) and DNA (genomic and amplicon-derived). Library complexity is assessed using qPCR prior to amplification. Illumina NGS technology uses adapter-ligated template molecules to populate a hollow glass flow cell. Individual target molecules are then amplified to create template clusters. Templates are sequenced using reversible, fluorescent-tagged terminator nucleotides. Following incorporation and image analysis, the fluorescent tags and terminators are removed and the next round of nucleotide incorporation is performed. The HiSeq 1000 has the capacity to create over 1.5 billion clusters across each lane in an 8-channel flow cell. With the capability of either single-end or paired-end sequence reads and 50 to 100 base read lengths, the HiSeq 1000 can generate up to 300 Gb from a single sequence run. Costs are reduced by indexing (“bar coding”) individual template libraries, allowing multiple libraries to be sequenced in each of the 8 flow cell lanes.

What can you do with Next Generation Sequencing?

 

Genomic DNA

RNA

How Does it Work? Libraries: Cluster Generation: Sequencing

Costs:

Library (RNA or DNA):  $150.00 per sample

Sequencing costs per lane:
Single End 50 base read                 $913.00
Single End 100 base read            $1,258.00
Paired End 50 base read              $1,563.00
Paired End 100 base read            $2,113.00      

Staff:

Jill K. Thompson
Steve G. Widen, PhD
Thomas G. Wood, PhD

Location: 6.142 Medical Research Building
Contact: tgwood@utmb.edu         Phone: 409-747-0387


Please contact the Core to discuss your sequencing project.