Research Interest:
Dr. Zhang was trained at UCSF Mass Spectrometry Facility under the
direction of Dr. Alma Burlingame, the chief editor of the Journal of
Molecular and Cellular Proteomics. His broad research interest focuses
on the development of state-of-the-art methodologies in mass
spectrometry and their applications in biology in the field of a
combination of proteomics, metabolomics and epigenetics, with the hope
of better understanding the cause of diseases and cancer. His current
research is to develop validated selective-reaction monitoring (SRM)
and parallel-reaction-monitoring (PRM) methods for quantification of
histone modifications in the studies spanned from epigenetic
reprogramming in replicating cells, in the processes of
receptor-mediated endocytosis and one-carbon metabolism, and in aging
and neurodegenerative diseases.
Publications:
- Kangling Zhang, Hui Tang, James Blankenship,
Patrick R. Jones, Peter Yau, and A. L. Burlingame. Identification of
acetylation and methylation sites of Histone H3 from chicken
erythrocite by MALDI-TOF, MALDI-PSD and nano-ESI tandem mass
spectrometry. Analytical Biochemistry 306: 259-269, 2002.
- Kangling Zhang, L. Huang, K. E. Williams, E. M.
Bradbury, P. Yau, Patrick R. Jones, M. J. Minch, A. L. Burlingame.
Histone acetylation and deacetylation: Analysis of histone H4
acetylation sites by mass spectrometry. Molecular and Cellular Proteomics 1: 500-508, 2002.
- Kangling Zhang*, Peter Yau, Brian S. Imai, Ron
New, E. M. Bradbury. Differentiation of Acetylated Peptides from
Methylated Peptides by Mass Spectrometry: An Important Application for
Determining Lysine 9 Acetylation and Methylation of Histone H3. Proteomics 4: 1-10, 2004.
- Kangling Zhang*, Joseph S. Siino, Patrick R.
Jones, Peter M. Yau, E. Morton Bradbury. A mass spectrometric “western
blot” to evaluate the correlations between histone acetylation and
methylation, Proteomics 4: 3765-3775, 2004.
- Mona Shahbazian, Kangling Zhang and Michael
Grunstein. Histone H2B ubiquitylation is dispensable for
mono-methylation but important for subsequent rounds of methylation by
Dot1 and Set 1. Molecular Cell 19: 271-277, 2005.
- Feng Xu, Kangling Zhang, Michael Grunstein, Acetylation in histone H3 globular domain regulates gene expression in yeast. Cell 121: 375-385, 2005.
- Catherine B. Miller, Feng Xu, Kangling Zhang, and Michael Grunstein. Acetylation of H2AZ Lys 14 is associated with genome-wide gene activity in yeast. Genes & Development 20: 711-722, 2006.
- Junhong Han, Hui Zhou, Bruce Horazdovsky, Kangling Zhang, Rui-Ming Xu, Zhiguo Zhang, Rtt109 acetylates histone H3 lysine 56 and regulates replisome stability. Science 315: 653-655, 2007.
- Vaniyambadi V. Sridhar, Avinish Kapoor, Kangling Zhang,
Jianjun Zhu, Tao Zhou, Paul M. Hasegawa, Ray A. Bressan and Jian-Kang
Zhu, Control of DNA methylation and heterochromatic silencing by
histone H2B deubiquitination. Nature 447: 735-738, 2007.
- Feng Xu, Qiongyi Zhang, Kangling Zhang, Wei
Xie, Michael Grunstein. Sir2 deacetylates histone H3 lysine 56 to
regulate telomeric heterochromatin structure in yeast. Mol. Cell. 27, 890-900, 2007.
- Kangling Zhang*, Vaniyambadi V. Sridhar,
Jianhua, Zhu, Avinish Kapoor, and Jian-Kang Zhu*. Mass spectrometry
reveals distinctive core histone post-translational modification
pattern in Arabidopsis thaliana. PLoS ONE 2 (e1210), 1-11, 2007.
- Gregory A. Horwiitz, Kangling Zhang, Matthew
A. McBrian, Michael Grunstein, Siavash, K. Kurdistani, and Arnold J.
Berk. Adenovirus e1a alters global patterns of histone modifications
for oncogenic transformation. Science 321: 1084-1085, 2008.
- Yi-Hui Lin, Purvi Kakadia, Ying Chen, Ya-Qiang Li, Aniruddha Deshpanda, Christian Buske, Kangling Zhang,
Yi Zhang, Guo-Liang, Xu, and Stefan K. Bohlander. Leukemic CALM-AF10
fusion causes global epigenetic change and promotes chromosomal
instability. Blood 114: 651-658, 2009.
- Bo Liu, Yi-Hui Lin, Agus Darwanto, Xue-Hui Song, Guo-Liang X, and Kangling Zhang. Identification and characterization of propionylation on histone H3 lysine 23 in mammalian cells. J. Biol. Chem. 284: 32288-32295, 2009.
- Jia-Lei Hu, Bo Zhou, Kangling Zhang, Jin-Qiu Zhou and Guo-Liang Xu. The N-terminus of histone H3 is required for de novo DNA methylation in chromatin. PNAS 106: 22187-22192, 2009.
- Agus Darwanto, Matthew Curts, Matthew,Schrag, Wolff Kirsch, Peng Liu, Guoliang Xu, Jonahan Neidigh, and Kangling Zhang. A modified “cross-talk” between histone H2B Lys-120 ubiquitination and H3 Lys-K79 methylation. J. Biol. Chem. 285: 21868-21876, 2010.
- Lei Xiong, Agus Darwanto, Seema Sharma, Jason Herring, Shaoyan
Hu, Maria Filippova, Valery Filippov, Yinsheng Wang, Chien-Shing Chen,
Penelope J. Duerksen-Hughes, Lawrence C. Sowers, and Kangling Zhang. Mass spectrometric studies on epigenetic interaction networks in cell differentiation. J. Biol. Chem. 286: 13657-13668, 2011.
- Yu-Fei He, Bin-Zhong Li, Zheng Li, Peng Liu, Yang Wang, Qingyu
Tang, Jianping Ding, Yingying Jia, Zhangcheng Chen, Lin Li,Yan Sun,
Xiuxue Li, Qing Dai, Chun-Xiao Song, Kangling Zhang, Chuan He, Guo-Liang Xu. Tet-Mediated Formation of 5-Carboxylcytosine and Its Excision by TDG in Mammalian DNA. Science 333:1303-1307, 2011.
- Kangling Zhang*, Matthew Schrag, Andrew Crofton, Harry Vinters, and Wolff Kirsch*. Targeted proteomics for quantification of histone acetylation in Alzheimer’s disease. Proteomics 12: 1261-1268, 2012.
- Weiqiang Qian, Daisuke Miki, Heng Zhang, Yunhua Liu, Xi Zhang, Kai Tang, Yunchao Kan, Honggui La, Xiaobing Shi, Kangling Zhang,
Xuemei Chen, Renyi Liu, Zhizhong Gong, Jian-Kang Zhu. A novel histone
acetyltransferase recognizes methylated DNA and unmethylated H3K4 marks
and regulates active DNA demethylation in Arabidopsis. Science 336:1445-1448, 2012.
- Hui Tang, Saied Mirshahidi, Maheswari Senthil, Kevork Kazanjian, Chien-Shing Chen, and Kangling Zhang.
Down-regulation of LXR/RXR activation and negative acute phase
response pathways in colon adenocarcinoma revealed by proteomics and
bioinformatics analysis. Cancer biomarkers, in press, 2014.
- Hui Tang, Huasheng Fang, Eric Yin, Allan R. Brasier, Lawrence C. Sowers, Kangling Zhang. Multiplexed parallel reaction monitoring (PRM) targets histone modifications on the Q-Exactive mass spectrometer. Anal. Chem. 86: 5526-5534, 2014