Sealy Center for Molecular Medicine

Research Interests

My research focuses on developing and applying of state-of-the-art mass spectrometry and proteomics to elucidate protein structure and function, to accurately quantify the temporal changes in the proteome, and to identify the potential diagnostic markers and drug targets for the treatment of diseases. Specifically, I am interested in the following aspects in mass spectrometry and proteomics:

1. Zhao Y, Zhang Y, Sun H, Maroto R and Brasier AR. Selective affinity enrichment of nitrotyrosine-containing peptides for quantitative analysis in complex samples. J Proteome Research. 2017, in press.
2. Zhang Y, Sun H, Zhang J, Brasier AR, and Zhao Y.  Quantitative assessment of the effects of trypsin digestion methods on affinity purification-mass spectrometry-based protein-protein interaction analysis. J Proteome Research. 2017, in press.
3. Tian B, Yang J, Zhao Y, Ivanciuc T, Sun H, Garofalo RP, Brasier AR. Bromodomain Containing 4 (BRD4) Couples NFkB/RelA With Airway Inflammation And The IRF-RIG-I Amplification Loop In Respiratory Syncytial Virus Infection Journal of Virology. 2017;in press:PMID: 28077651. doi: doi: 10.1128/JVI.00007-17
4. Ijaz T, Jamaluddin M, Zhao Y, Zhang Y, Finnerty CC, Jay J, Herndon D, Tilton R, Brasier AR. Coordinate activities of BRD4 and CDK9 in the transcriptional elongation complex are required for TGFb-induced Nox4 expression and myofibroblast transdifferentiation Cell Death Differ. 2017;;8(2)::e2606. doi: 10.1038/cddis.2016.434.
5. Zhao Y, Jamaluddin M, Zhang Y, Sun H, Ivanciuc T, Garofalo RP, Brasier AR. Systematic Analysis of Cell-Type Differences in the Epithelial Secretome Reveals Insights into the Pathogenesis of Respiratory Syncytial Virus-Induced Lower Respiratory Tract Infections. Journal of immunology (Baltimore, Md : 1950). 2017;198(8):3345-64. doi: 10.4049/jimmunol.1601291.
6. Tian B, Zhao Y, Sun H, Zhang Y, Yang J, Brasier AR. BRD4 Mediates NFkB-dependent Epithelial-Mesenchymal Transition and Pulmonary Fibrosis via Transcriptional Elongation. The American Journal of Physiology -Lung Cellular and Molecular Physiology 2016;311(6):L1183-L201. doi: 10.1152/ajplung.00224.2016;
7. Zhao Y, Tian B, Sadygov RG, Zhang Y, Brasier AR. Integrative proteomic analysis reveals reprograming tumor necrosis factor signaling in epithelial mesenchymal transition. J Proteomics. 2016;148:126-38. Epub 2016/07/28. doi: 10.1016/j.jprot.2016.07.014.
8. Desai P, Yang J, Tian B, Sun H, Kalita M, Ju H, Paulucci-Holthauzen A, Zhao Y, Brasier AR, Sadygov RG. Mixed-effects model of epithelial-mesenchymal transition reveals rewiring of signaling networks. Cell Signal. 2015;27(7):1413-25. Epub 2015/04/12. doi: 10.1016/j.cellsig.2015.03.024.
9. Brasier AR, Zhao Y, Wiktorowicz JE, Spratt HM, Nascimento EJ, Cordeiro MT, Soman KV, Ju H, Recinos A, 3rd, Stafford S, Wu Z, Marques ET, Jr., Vasilakis N. Molecular classification of outcomes from dengue virus -3 infections. Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology. 2015;64:97-106. Epub 2015/03/03. doi: 10.1016/j.jcv.2015.01.011.
10. Yang J, Zhao Y, Kalita M, Li X, Jamaluddin M, Tian B, Edeh CB, Wiktorowicz JE, Kudlicki A, Brasier AR. Systematic Determination of Human Cyclin Dependent Kinase (CDK)-9 Interactome Identifies Novel Functions in RNA Splicing Mediated by the DDX5/17 RNA Helicases. Molecular & Cellular Proteomics : MCP. 2015. Epub 2015/07/26. doi: 10.1074/mcp.M115.049221.
11. Brasier AR, Zhao Y, Spratt HM, Wiktorowicz JE, Ju H, Wheat LJ, Baden L, Stafford S, Wu Z, Issa N, Caliendo AM, Denning DW, Soman K, Clancy CJ, Nguyen MH, Sugrue MW, Alexander BD, Wingard JR. Improved Detection of Invasive Pulmonary Aspergillosis Arising during Leukemia Treatment Using a Panel of Host Response Proteins and Fungal Antigens. PLoS ONE. 2015;10(11):e0143165. doi: 10.1371/journal.pone.0143165.
12. Zhao Y, Valbuena G, Walker DH, Gazi M, Hidalgo M, De Sousa R, Oteo JA, Goez Y, Brasier AR. Endothelial cell proteomic response to Rickettsia conorii infection reveals activation of the Jak-STAT-ISG15 pathway and reprogramming plasma membrane integrin/cadherin signaling. Molecular & Cellular Proteomics. 2015. doi: 10.1074/mcp.M115.054361.