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Nex-Gen Core: Next Generation Sequencing Core Facility

UTMB’s Next Generation Sequencing (NGS) Core utilizes an Illumina HiSeq 1500 sequencing system to perform massively parallel sequencing for genetic analysis. The NGS Core offers support in library construction from various template sources; RNA (total, poly A+ and miRNA), chromatin immunoprecipitated DNA (ChIP-Seq) and DNA (genomic and amplicon-derived). Library complexity is assessed using qPCR prior to amplification. Illumina NGS technology uses adapter-ligated template molecules to populate a hollow glass flow cell. Individual target molecules are then amplified to create template clusters. Templates are sequenced using reversible, fluorescent-tagged terminator nucleotides. The HiSeq 1500 has the capacity to create over 1.5 billion clusters across each lane in an 8-channel flow cell. The 1500 HiSeq offers the versatility of using either an eight lane or a two lane flow cell. With the capability of either single-end or paired-end sequence reads and 50 to 100 base read lengths, the HiSeq 1500 can generate up to 300 Gb from a single sequence run. The two lane platform can also accommodate 150 base reads. Costs are reduced by indexing (“bar coding”) individual template libraries, allowing multiple libraries to be sequenced in each of the flow cell lanes. Investigators have the option of two-lane, eight-lane or first available in selecting the platform for their sequencing project.

What can you do with Next Generation Sequencing?
  • ChIP-Seq
  • De novo assembly
  • Variant identification
  • SNPs
  • Transcriptome (reference-guided or de novo)
  • miRNA and small ncRNA sequencing
  • Quantitative - RNA-Seq expression analysis

Next Generation Sequencing Costs: September 1, 2017

Library Cost

PlatformRun TypeEstimated ReadsCost
Full 8PE 50 base150M$1,770.00
RapidSE 50 base240M$1,891.00
 SE 100 base240M$2,491.00
 SE 150 base240M$3,091.00
 PE 50 base240M$2,991.00
 PE 100 base240M$3,836.00
NxSeq550SE 150 base130M$1,244.00
 PE 75 base130M$1,244.00
 PE 150 base130M$1,884.00
 SE 75 base400M$1,910.00
 PE 75 base400M$3,240.00
 PE 150 base400M$4,910.00
MiniSeq75 cycles25M$925.00
 150 cycles25M$1,065.00
 300 cycles25M$1,645.00
 300 cycles8M$650.00
M, million; SE, single end; PE, paired end
 
LibraryCost
RNA-Seq Non-Stranded, no dT selection              $223.00
RNA-Seq Non-Stranded & dT Selected                  $230.00
RNA-Seq Stranded & dT Selected                       $238.00
 rRNA Depletion  (additional cost)                      $88-98.00
ChIP-Seq                                                               $203.00
DNA-Seq (Covaris)                                               $245.00
DNA-Seq  Nextera                                                $259.00
microRNA (Small RNA)                                       $220.00

Staff:

Jill K. Thompson
Steve G. Widen, PhD
Thomas G. Wood, PhD

Location: 6.142 Medical Research Building
Contact: tgwood@utmb.edu
Phone: (409)747-0387

Please contact the Core to discuss your sequencing project.